|
KMID : 0379519980140040507
|
|
한국독성학회지
1998 Volume.14 No. 4 p.507 ~ p.513
|
|
|
Cytosolic Calcium Alteration and Cell Injury by Silica in Rat Hepatocytes
|
|
Cha Seok-Ho
Cha Shin-Woo
Ko Chang-Bo
Yu Soung-Roung
Kim Hye-Sun
Paik Sang-Gi
|
|
|
Abstract
|
|
|
|
The purpose of this study was to clarify the effect of silica on cytosolic free calcium mobilization and cell injury in primary cultured rat hepatocytes. Cytosolic free calcium concentration ([Ca^{2+}]) was measured employing calcium sensitive fluorescent dye, Fura-2 / AM, and cell injury was evaluated by determination of cellular ATP contents. Silica increased [Ca^{2+}], in a concentration-dependent manner in hepatocytes (10^{-5} ~10^{-2} M). Silica caused a biphasic increase in [Ca^{2+}], which was composed of an initial rapid rise and following sustained phase. Ca^{2+} removal from the medium resulted in abolishment of initial and sustained phase of silica (10^{-2} M)-induced [Ca^{2+}], in hepatocytes. The pretreatment with nifedipine (1 muM) attenuated silica-induced [Ca^{2+}], increases. Silica decreased cellular ATP contents in a dose-dependent manner. This silica-induced cell injury was attenuated by the pretreatment with EGTA (100 muM) and nifedipine (1 muM). This study suggests that the elevation of [Ca^{2+}], caused by silica may be due mainly to influx through a plasma membrane Ca^{2+} channel and hepatotoxicity by silica relate with alteration of calcium homeostasis.ium homeostasis.
|
|
|
KEYWORD
|
|
|
Silica, Cytosolic free calcium, Cell injury, Hepatocyte, Cellular ATP content
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|